3.
Coloration
This is the most obvious, and the first thing anyone notices about a box turtle.
The eyes of a male are usually bright red. Males will also tend to be brightly colored, especially around the head, neck and front legs.
Females will tend to have dark brown to reddish brown eyes, and are usually the drabber sex.
This is the least reliable method of sex determination.
I have seen females with red eyes, and some are quite colorful.